NIJISANJI (main branch)

On 11 January, Ichikara Inc. announced that it was recruiting "Official Virtual Livers" to serve as representatives for its iPhoneX Live2D streaming app, NIJISANJI. On 8 February, Ichikara Inc. officially announced the debut of eight "Virtual YouTubers" on Mirrativ and YouTube using the NIJISANJI app. These eight would later become known as NIJISANJI's first generation of Virtual Livers. On 15 March, Ichikara Inc. officially announced NIJISANJI's second generation of ten Virtual Livers, formalizing "NIJISANJI" as the agency's name for the first time. On 26 April, Ichikara Inc. announced that it was developing 3D models for NIJISANJI Livers to stream with, starting with Tsukino Mito. On 2 May, Ichikara Inc. introduced NIJISANJI Gamers, a new Virtual Liver group with a focus on Let's Play streams, with Kanae and Akabane Youko being its founding members. On 5-15 May, Ichikara Inc. held auditions for NIJISANJI SEEDs, a third Virtual Liver group in the NIJISANJI Project, with applicants being considered "regardless of experience or skill". The first batch of selected candidates, later known as the first generation of SEEDs, started their activities on 3 June, On 6 July, three new Livers joined NIJISANJI Gamers: Sasaki Saku, Yamiyono Moruru, and Honma Himawari. On 30 July, four new Livers joined NIJISANJI Gamers: Makaino Ririmu, Kuzuha (formerly an independent VTuber), Setsuna, and Shiina Yuiika. On 9 August, the first batch of NIJISANJI SEEDs' second generation began their activities. On 31 August, the second batch of NIJISANJI SEEDs' second generation began their activities. On 13 September, Ichikara Inc. announced that it would begin implementing a 2.0 update to NIJISANJI's Live2D models. The updates, now referred to as "2.0 Brushups", are designed to improve a Liver's degree of movement and expand their range of expressions. On 25 September, the third batch of NIJISANJI SEEDs' second generation began their activities. On 13 December, Ichikara Inc. announced that both NIJISANJI Gamers and NIJISANJI SEEDs would be merged into the main NIJISANJI group by the end of the month. After the merge, Livers originally from Gamers or SEEDs tend to use the term "ex-Gamers/SEEDs" for collabs with their debut-mates.

On 8 January, NIJISANJI announced a new wave of two Livers. The new wave, together known as "Kouhaku Aho Gassen", began their streaming activities on 10 January. On 17 January, NIJISANJI announced a new wave of two Livers. The new wave, together known as "MireRoa" or "Usa-chan Club", began their streaming activities on 20 January. On 28 January, NIJISANJI announced a new wave of three Livers. The new Livers (Onomachi Haruka, Kataribe Tsumugu, and Seto Miyako) began their streaming activities on 31 January. On 8 March, NIJISANJI announced a new wave of two Livers. The new Livers (Shindo Raito and Otogibara Era) began their streaming activities on 10 March. On 22 March, NIJISANJI announced a new all-female wave of three Livers. The new wave, together known as "Sanbaka", began their streaming activities on 23 March. On 2 April, NIJISANJI announced a new wave of two Livers. The new wave, together known as "Beni Zuwaigani", began their streaming activities on 7 April. On 29 April, NIJISANJI announced a new wave of two Livers. The new wave, unofficially known as "MahiLulu", began their streaming activities on 1 May. On 17 May, NIJISANJI announced a new wave of two Livers. The new wave, together known as "LvEx", began their streaming activities on 21 May. On 19 June, NIJISANJI announced a new wave of two Livers. The new wave, together known as "Chichiyama", began their activities on 25 June. On 3 July, NIJISANJI announced a new wave of three Livers. The new wave, together known as "SMC-gumi", began their activities on 6 July. On 19 July, Ichikara Inc. announced a new branch of the NIJISANJI Project in Indonesia called "NIJISANJI ID". Auditions for four Virtual YouTuber positions were open from 19 July until 4 August, and the three selected candidates officially began their activities on 13 September. On 24 July, NIJISANJI announced a new wave of three Livers. The new wave, together known as "Blues", began their activities on 28 July. On 8 August, NIJISANJI announced a new, all-female wave of three Livers. The new wave, together known as "Posanke", began their activities on 13 August. On 19 September, NIJISANJI announced a new wave of three Livers. The new wave, together known as "Tulip-gumi", began their activities on 21 September. On 17 October, NIJISANJI announced a new wave of three Livers. The new wave, together known as "Orihimeboshi", began their activities on 16 October. On 31 October, NIJISANJI announced a new wave of three Livers. The new wave, together known as "Aka no Soshiki", began their activities on 3 November. On 28 November, NIJISANJI announced a new wave of three Livers. The new wave, together known as "Night Kingdom", began their activities on 30 November. On 8 December, NIJISANJI held a concert, "Virtual to LIVE in Ryogoku Kokugikan 2019", themed around its 1st anniversary song "Virtual to LIVE". A total of 15 Livers performed at Ryogoku Kokugikan in Tokyo to a live audience of about 6,000, and the online broadcast received over 21,000 comments on Niconico. During the concert, NIJISANJI announced the formation of the unit "petit fleurs", consisting of Morinaka Kazaki and Otogibara Era. The new unit would be signed with NBC Universal Entertainment. On 17 December, Ichikara Inc. announced a new branch of the NIJISANJI Project in South Korea called "NIJISANJI KR". Auditions for five virtual YouTuber positions were open from 20 December until 6 January 2020, and the three selected candidates officially began their activities in late January 2020. On 18 December, the second wave of NIJISANJI ID started their activities. On 26 December, NIJISANJI announced a new wave of three Livers. The new wave, together known as "Mananatsu", began their activities on 29 December.

On 30 January, NIJISANJI announced a new wave of three Livers. The new wave, together known as "Meifu", began their activities on 1 February. From 9 February to 5 April, NIJISANJI held a 6-show national music concert tour called "NIJISANJI JAPAN TOUR 2020 Shout in the Rainbow!". A total of 29 NIJISANJI members performed across 5 Zepp venues (Sapporo, Fukuoka, Namba, Nagoya, and DiverCity Tokyo). Unfortunately, due to the COVID-19 pandemic, the Zepp DiverCity Tokyo show was entirely cancelled and the second Zepp Namba show was exclusively streamed online. On 13 March, the third wave of NIJISANJI ID started their activities. On 2 April, NIJISANJI announced a new, all-male wave of three Livers with a Japanese theme. The new wave, together known as "VΔLZ", began their activities on 4 April. On 3 April, NIJISANJI announced a new program on its main channel, "Yashiro's and Sasaki's LeverGacha Daipan" (ヤシロ&ササキのレバガチャダイパン). This program would consist of a weekly gaming/variety talk show hosted by Yashiro Kizuku and Sasaki Saku in 3D, featuring other Livers as guests, and would be broadcast every Friday at 19:00 JST, starting on 10 April. On 22 May, the second wave of NIJISANJI KR started their activities. On 30 June, NIJISANJI announced a new wave of two female Livers. The new wave, together known as "KiraMero", began their activities on 11 July. On 3 July, NIJISANJI's official YouTube channel opened memberships. On 31 July, the third wave of NIJISANJI KR started their activities. On 6 August, NIJISANJI announced a new, all-female wave of five Livers with a high school theme. The new wave, together known as "Selene Drama Club", began their activities on 9 August. On 7 August, the fourth wave of NIJISANJI ID started their activities. On 9 October, the fourth wave of NIJISANJI KR started their activities. On 11 November, the fifth wave of NIJISANJI ID started their activities. On 21 December, NIJISANJI announced a new program on its main channel, "B-Grade Variety of NIJISANJI (tentative)" (にじさんじのB級バラエティ(仮)), a variety show hosted by Ibrahim and Hayase Sou happening every other week. This program would be broadcast every other Tuesday at 24:00 JST, starting on 22 December.

On 28 February, it was announced at NIJISANJI Festival (NIJIFes) 2021 that NIJISANJI would begin implementing 3.0 Brushups, designed to significantly improve a Liver's degree of movement and allow for custom facial expressions. JK-gumi (Tsukino Mito, Higuchi Kaede, Shizuka Rin) would be the first to receive 3.0 Brushups in April. Additionally, NIJISANJI ID's first wave (Hana Macchia, ZEA Cornelia, and Taka Radjiman) received 2.0 Brushups during the festival. During the closing ceremony, NIJISANJI also announced that the unit "▽▲TRiNITY▲▽" (Takamiya Rion, Furen E Lustario and Hakase Fuyuki) had signed a music deal with NBCUniversal Entertainment. On 3 March, Kuzuha became the most subscribed Liver in NIJISANJI, surpassing Tsukino Mito, who had previously held the highest subscriber count since 2018. On 23 April, the fifth wave of NIJISANJI KR started their activities. On 12 May, the English version of NIJISANJI's official website was released. On 13 May, NIJISANJI opened its official TikTok account. On 19 July, NIJISANJI announced a new wave of five Livers, the main branch's first debuts in nearly a year. The new wave, together known as "Eden-gumi", started their activities on 22 July. On 28 July, the sixth wave of NIJISANJI ID started their activities. On 12-15 August, NIJISANJI held their Power Pro 2020 (パワプロ2020, Pawapuro 2020) tournament, "NIJISANJI Koshien Summer 2021" (にじさんじ甲子園 2021夏). This four-day event featured Livers from all NIJISANJI branches (excluding VirtuaReal), hosted by Maimoto Keisuke and Tenkai Tsukasa. The finals reached over 200,000 concurrent viewers, the highest for any NIJISANJI stream on YouTube. On 25 August, the sixth wave of NIJISANJI KR started their activities. On 2 September, Kuzuha reached 1 million subscribers, becoming the first NIJISANJI Liver and first male VTuber worldwide to do so. On 21 October, the NIJISANJI YouTube channel announced the formation of the unit "ROF-MAO" (ろふまお), consisting of Kagami Hayato, Kenmochi Touya, Fuwa Minato, and Kaida Haru. The new unit has its own official website, YouTube channel and Twitter account, and would regularly hold a program called "ROF-MAO Juku" (ろふまお塾, ROF-MAO Cram School) on its official channel. On 31 October, the NIJISANJI Official Twitter account announced that NIJISANJI 4th Anniversary LIVE "FANTASIA" would be held on 22 and 23 January 2022. The two-day concert would feature 16 Livers in 3D with matching outfits.

On 20 January, NIJISANJI announced that Day 2 of its 4th anniversary concert, "FANTASIA," would be cancelled due to Kenmochi Touya, Kanae, Kagami Hayato, and Yashiro Kizuku testing positive for COVID-19. The following day, NIJISANJI further announced that Day 1 of "FANTASIA" would also be cancelled due to the worsening COVID-19 situation in Japan. On 17 February, ANYCOLOR announced that the NIJISANJI ID and NIJISANJI KR branches would be merged with the main NIJISANJI branch in early April 2022. Currently active Livers would continue their activities under the NIJISANJI brand, using any languages of their choice. All activities and events on the ID and KR social media channels would be cancelled, including currently running programs and new wave auditions. On 16 March, NIJISANJI announced a new all-female wave of three Livers, "Ranunculus", originally from VIRTUAL TALENT ACADEMY (VTA). "Ranunculus" began their activities on 20 March, releasing their original song, "DONBURA KONBURA SPEAKERS". They were the first VTA graduates to debut directly into NIJISANJI, as well as the main branch's first wave to debut with an original song and predetermined unit name. On 15 April, NIJISANJI ID and NIJISANJI KR officially merged into the main branch. On 15 April, NIJISANJI announced the major rerelease of NIJISANJI JP Livers' "Welcome Goods & Voice" which include voice pack, can badge, and acrylic stand staring from 19 April. On 19 April, NIJISANJI announced a renewal of "NIJISANJI no Happy Hour!!" (にじさんじのハッピーアワー!!) into "NIJISANJI no TOYBOX!" (にじさんじのTOYBOX！). Hosted by Sasaki Saku, Axia Krone, and Eli Conifer, the new program would start airing on 17 May 2022 (20:00 JST) and the first episode would be free to watch. On 24 April, the NIJISANJI official YouTube channel reached 1 million subscribers. On 3 May, NIJISANJI announced a new program to its main channel called "Game-Ru? Game-Lu!", replacing the previous Levergacha program that ended in April. This program will be broadcast every Wednesday 20:00 JST beginning on 4 May as an official game live program hosted by Amamiya Kokoro, Matsukai Mao and Yorumi Rena. On 5 May, NIJISANJI announced the "NIJISANJI 4th Anniversary LIVE FANTASIA" concert was rescheduled to 30 September and 1 October as part of NIJISANJI Festival (NIJIFes) 2022's "Night Stage" #にじフェス2022. On 14 May, Kanae reached 1 million YouTube subscribers, making him the second NIJISANJI Liver and male VTuber worldwide to reach the milestone after Kuzuha. On 21 May, NIJISANJI announced the debut of a new NIJISANJI Liver, Hyakumantenbara Salome. She was the first solo debut in the agency. Salome began her streaming activities on 24 May. On 7 June, Hyakumantenbara Salome reached 1 million subscribers in just under 14 days since her debut, making her the fastest NIJISANJI Liver NIJISANJI and VTuber worldwide to reach the milestone. She is the third NIJISANJI Liver to reach 1 million after Kuzuha and Kanae, and the first female Liver to do so. On 20 June, Salome became the most subscribed Liver in NIJISANJI, surpassing Kuzuha, who had previously held the highest subscriber count since March 2021. On 23 June, NIJISANJI announced the formation of the unit "Nornis", consisting of Machita Chima, Inui Toko, and Asahina Akane, with their single "Abyssal Zone". This new unit would be signed with the record label Virgin Music. On 13 July, NIJISANJI announced a new, all-male wave of four Livers, "VOLTACTION", originally graduates from VTA. A peel-off advertisement for the new unit was featured at Shibuya Station from 11 July to 17 July. "VOLTACTION" began their activities on 16 July, releasing their original song "INLAID". They were the first wave in the main branch to debut with a unit-dedicated YouTube channel and Twitter account. On 5 August, NIJISANJI announced FOCUS ON - NIJISANJI Single Collection -, a project where each month two different Livers will have a singular single CD album released in the market as a physical and digital. Beginning in October 2022, the project continues going a year later in 2023. On 6 August, NIJISANJI's official Twitter announced that that multiple ex-ID and ex-KR Livers would participate in NIJIFes 2022, including a special 3D showcase from Hana Macchia. On 11-14 August, NIJISANJI held their Power Pro 2022 tournament, "NIJISANJI Koshien 2022" (にじさんじ甲子園2022). This four-day event featured Livers from all across NIJISANJI (excluding VirtualReal), and was hosted by Maimoto Keisuke and Tenkai Tsukasa. The event was sponsored by Rakuten Collection, and the finals stream reached over 320,000 concurrent viewers, breaking the previous year's record of 201,000. On 29 September, NIJISANJI's Korean and Indonesian Twitters announced that multiple ex-ID and ex-KR Livers scheduled to appear at NIJIFes 2022 would be unable to participate due to "unforseen circumstances." These circumstances were later revealed to be visa processing issues. On 1-2 October, Hana Macchia showcased her 3D model on Day 2 of NIJIFes 2022, becoming the first ex-ID Liver to receive 3D. It was also announced that NIJISANJI would begin holding 3D debuts for overseas Livers in the near future. On 19-20 November, NIJISANJI Festival 2022 (にじさんじ遊戯王マスターデュエル祭2022) took place across 2 days on NIJISANJI Official YouTube channel (Day 1 & Day 2). Six male and six female Livers participated in the event: Yashiro Kizuku, Kagami Hayato, Ibrahim, Mashiro Meme, Maimoto Keisuke, Kuzuha, Kurusu Natsume, Matsukai Mao, Hoshikawa Sara, Yorumi Rena, Fumi, and Yamagami Karuta. On 12 December, Tsukino Mito reached 1 million subscribers, making her the fourth NIJISANJI Liver and second female Liver to reach the milestone. On 29-31 December, "NIJISANJI Unit Music Festival 2022" (にじさんじユニット歌謡祭2022) took place across 3 days on the official NIJISANJI YouTube channel. Formerly a Liver-organized project, the event featured over 80 Livers performing in 3D in various units, with Rindou Mikoto and Kenmochi Toya serving as MCs. Day 1 was free to watch, while Day 2 and Day 3 were paid admission. The entirety of Day 1, as well as free parts of Days 2 and 3, were later reuploaded in a single compilation on 22 January 2023.

On 16 January, NIJISANJI announced a new, all-female wave of seven Livers, three of who were graduates originally from VTA while the other four were from the advanced course of VTA which doesn't stream on public. The new wave, together known as "Idios", began their streaming activities on 19-20 January. On 29 March, NIJISANJI announced a new, all-male wave of four Livers, two of who were graduates originally from VTA while the other two were from advanced courses from VTA, without streaming in public. The new wave debuted on 2 April. On 3 April, NIJISANJI announced a new program project for its main channel, "NIJIBAN". The new program would air weekly on Tuesdays at 22:00 JST, and unlike existing programs, its content would change each month. The first NIJIBAN program, "Nagao & Petra's We are NIJISANJI" (長尾とペトラのたいして変わらん), would be hosted by Nagao Kei and NIJISANJI EN's Petra Gurin, and would air for four weeks starting on 4 April. On 3 April, NIJISANJI announced the formation of the unit "Nanajigen Seitokai" (七次元生徒会!), consisting of Kanae, Saegusa Akina, Leos Vincent, Higuchi Kaede, Ryushen, and Suo Sango. The unit would air a new program on it's own YouTube channel every Wednesday at 19:00 JST, with a goal to reach the support of 1 million parents and students. On 26 April, NIJISANJI announced a new all-male wave of four Livers following the same theme as the previous male wave with heroes. Two of the members were graduates originally from VTA while the other two were from advanced courses from VTA, without streaming in public. The new wave debuted on 29 April. On 28 April, NIJISANJI announced that the musical unit Raindrops would have its activities suspended on May 1. On 4 August, NIJISANJI announced the stages and members participating on NIJISANJI Festival (NIJIFES), which is going to happen in 23 and 24 December. Alongside NIJIFES itself, special events to happen in it like 5th Anniversary concert called Symphonia featuring JP members, a Kuzuha solo stage (Kaleidoscope) and Sakuyui teather (Shiina Yuika and Sasaki Saku) were announced. On 2 October, ANYCOLOR announced the Theme Song of NIJISANJI and NIJISANJI EN Heroes Oriens, Dytica and Krisis, called "SUPER DUPER". Unlike debut songs, this original song had Higuchi Kaede from NIJISANJI and Ren Zotto from NIJISANJI EN singing it instead of the own Livers represented. On 21 November, NIJISANJI announced a new mixed wave of three Livers, the first mixed wave in NIJISANJI since Edengumi debut in 2021. All of the members were originally graduates from VTA. The new wave debuted on 24 November. On 30 December, Hoshikawa Sara reached 1 million subscribers, making her the fifth NIJISANJI Liver and third female Liver to reach the milestone.

On 12 March, NIJISANJI announced a new, all-male wave of three Livers, two of who were graduates originally from VTA. They were set to debut on 15 March. On 26 April, the unit "Gakudan V!VO" (楽団V!VO) began their activities. Unlike NIJISANJI's previous vocal-based musical units, Gakudan V!VO is a chamber orchestra, consisting of pianist Suzuki Masaru, violinist Seraph Dazzlegarden, flautist Eli Conifer, cellist Emma★August, and trombonist Tachitsute Toto. The group streams practice sessions live twice monthly in rotation on the members' channels, with the goal of performing in front of a live audience on a big stage.